| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 821 | Methanosphaera stadtmanae | GCAAACAACTCTGGAGCAACA | AGAGTCCTGTATCCCCTGCA | 59.59 | 59.66 | 259 | 37 |
100.00%
|
100.00%
|
| Primer 822 | Methanosphaera stadtmanae | CCCATATCAACAGCTGAAGGC | TGGTATCTTGCCTCATGGGG | 59.05 | 59.15 | 182 | 37 |
100.00%
|
100.00%
|
| Primer 823 | Methanosphaera stadtmanae | GGTCCAACACCTCTTGCATCT | TTGTATTTAGAAGCGCAGGGG | 60.27 | 58.36 | 178 | 37 |
100.00%
|
100.00%
|
| Primer 824 | Methanosphaera stadtmanae | TGGTGATGCCACATCAGTTCA | TCTGTTGAACCCCTACCAAGA | 59.93 | 58.30 | 297 | 36 |
100.00%
|
100.00%
|
| Primer 825 | Methanosphaera stadtmanae | TTGCACAGCAGGAACCTCA | AGCAATTGCAGGCCAAATAGA | 59.47 | 58.54 | 254 | 36 |
100.00%
|
100.00%
|
| Primer 826 | Methanosphaera stadtmanae | ACACCCCTTGTTCTTGATCCA | TGCACCAGAAACTGCAATTGT | 59.22 | 59.24 | 282 | 35 |
100.00%
|
100.00%
|
| Primer 827 | Methanosphaera stadtmanae | TGCTAGGATATCTGGAGCCCA | CTGCCACAGCCATAATTGCA | 59.85 | 59.18 | 278 | 35 |
100.00%
|
100.00%
|
| Primer 828 | Methanosphaera stadtmanae | TGGTGGAATTGCTCAGCGT | CCACCACTAGGCGTGTCTT | 59.93 | 59.33 | 195 | 34 |
100.00%
|
100.00%
|
| Primer 829 | Methanosphaera stadtmanae | ACATGCTGGTCTTTCCATTCC | ATCAGGTTGTCTCATAGGCCT | 58.55 | 58.23 | 172 | 33 |
100.00%
|
100.00%
|
| Primer 830 | Methanosphaera stadtmanae | CATGCTGGTCTTTCCATTCCT | ATCAGGTTGTCTCATAGGCCT | 58.27 | 58.23 | 171 | 33 |
100.00%
|
100.00%
|